H3K4me2 Monoclonal Antibody (T.767.5)/H3K4me2单克隆抗体（T.767.5）
货号：MA5-14977
规格：100 µL
价格：4880
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Synthetic peptide corresponding to the amino termi
物种：其它
宿主：兔
抗体亚型：IgG
克隆号：T.767.5
荧光染料：其它
| 类型: | 单抗 | 同型对照： | |
| 浓度： | | 用法： | 1:250(ICC)；1:250(IF)；1:1500(IHC (P))；1:25(IP)；1:1000(WB) |
产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with di-methylated or tri-methylated histone H3 Lys9, Lys27, Lys36 or histone H4 Lys20.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K4me2 Antibody (MA5-14977) in IFImmunofluorescence analysis of Di-Methyl-Histone H3 (Lys4)was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Monoclonal Antibody (Product # MA5-14977) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K4me2 Antibody (MA5-14977) in IFImmunofluorescent analysis of Di-Methyl-Histone H3 (Lys4) using a monoclonal antibody (Product # MA5-14977). |
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