H3K9me2 Polyclonal Antibody/H3K9me2多克隆抗体
货号：PA5-16195
规格：100 µL
价格：4880
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Synthetic peptide corresponding to the amino termi
物种：其它
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | | 用法： | 1:250(ICC)；1:250(IF)；1:25(IP)；1:1000(WB) |
产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with di-methylated or tri-methylated histone H3 Lys27.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K9me2 Antibody (PA5-16195) in IFImmunofluorescence analysis of Di-Methyl-Histone H3 (Lys9)was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # PA5-16195) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K9me2 Antibody (PA5-16195) in WBWestern blot analysis was performed on HeLa (Lane 1), HCT 116 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4) and JURKAT (Lane 5). The blot was probed with Anti- Di-Methyl-Histone H3 (Lys9) (Product #PA5-16195, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Di-Methyl-Histone H3 (Lys9) was observed across the cell lines tested. |
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| 参考文献： |
| 1. Nature communicationsThe cell-wide web coordinates cellular processes by directing site-specific Ca2+flux across cytoplasmic nanocourses. |
QQ:1749072012