H3K79me3 Polyclonal Antibody/H3K79me3多克隆抗体
货号：49-1020
规格：50 µg
价格：4809
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：raised in rabbits against histone H3 containing th
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | 0.6 mg/mL | 用法： | 1-10 µg/1x10^6 cells(ChIP)；1:100,000(DB)；1:500(ELISA)；1:1,000(WB)；1 µg per assay(ChIP-Seq) |
靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K79me3 Antibody (49-1020) in ChIPChIP assays were performed using HeLa cells, the anti-H3K79me3 antibody (Product # 49-1020), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and various amounts of anti-H3K79me3 antibody (1, 2, 5, and 10 µg). IgG (5 µg⁄IP) was used as a negative IP control. qPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. H3K79me3 shows a preference for active promoters. Therefore the promoter of GAPDH, a housekeeping gene, was used as a positive control target. Exon 2 of myoglobin was used as a negative control target. This figure shows the recovery, expressed as a percentage of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis) of the GAPDH promoter and of myoglobin exon 2. H3K79me3 Antibody (49-1020) in ChIPChIP was performed with the anti-H3K79me3 antibody (Product # 49-1020) on sheared chromatin from 1 million HeLaS3 cells. A titration of the antibody consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (5 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for exon 2 of the inactive myoglobin gene. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters. |
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| 参考文献： |
| 1. The Journal of biological chemistryA novel disrupter of telomere silencing 1-like (DOT1L) interaction is required for signal transducer and activator of transcription 1 (STAT1)-activated gene expression.2. The Journal of biological chemistryA novel disrupter of telomere silencing 1-like (DOT1L) interaction is required for signal transducer and activator of transcription 1 (STAT1)-activated gene expression. |
QQ:1749072012