H3K9me3 Polyclonal Antibody/H3K9me3多克隆抗体
货号：PA5-31910
规格：100 µL
价格：5942
产品类型：抗体和染料
品牌：Thermo Fisher
抗原：Synthetic peptide corresponding to a region within
物种：人/小鼠/大鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay Dependent(ChIP)；1:100-1:1,000(ICC)；1:100-1:1,000(IF)；1:100-1:1,000(IHC (P))；Assay Dependent(IM)；1:100-1:1000(IP)；1:500-1:3,000(WB)；0.25 µg/mL(Array) |
产品详细信息Recommended positive controls: 293T, A431, HeLa, HepG2.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据： |
H3K9me3 Antibody (PA5-31910) in IFImmunofluorescence analysis of Tri-Methyl-Histone H3 (Lys9) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tri-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody (Product # PA5-31910) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K9me3 Antibody (PA5-31910) in IFImmunofluorescent analysis of Tri-Methyl-Histone H3 (Lys9) in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a Tri-Methyl-Histone H3 (Lys9) polyclonal antibody (Product # PA5-31910). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. |
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