Phospho-Histone H1 (Thr17) Polyclonal Antibody/磷酸化组蛋白H1（Thr17）多克隆抗体
货号：PA5-40288
规格：100 µL
价格：4532
产品类型：抗体和染料
品牌：Alfa Aesar
抗原：Synthesized peptide derived from human Histone H1
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 多抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 1.5 µg/1x10^6 cells(ChIP)；5 µg/mL(ICC)；5 µg/mL(IF)；1:500-1:2000(WB) |
产品详细信息This antibody is predicted to react with mouse and monkey based on sequence homology.靶标信息This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift so it is unlikely to encode a functional protein.
| 数据： |
Phospho-Histone H1 (Thr17) Antibody (PA5-40288) in ICCImmunofluorescence analysis of Phospho-Histone H1 (Thr17) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Histone H1 (Thr17) Rabbit Polyclonal Antibody (Product # PA5-40288) at 5 microgram/mL concentration in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. Phospho-Histone H1 (Thr17) Antibody (PA5-40288) in WBWestern blot analysis of SKOV3 cells using an anti-HIST1H1B polyclonal antibody (Product # PA5-40288). |
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