UBE2B Polyclonal Antibody
货号：PA5-21316
规格：100 µL
价格：4880
产品类型：标签抗体
品牌：Thermo Fisher
抗原：Recombinant protein fragment contain a sequence co
物种：人/小鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 一抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 1:100-1:1000(ICC);1:100-1:1000(IF)；1:500-1:10,000(WB) |
产品详细信息Recommended positive controls: mouse brain, 3xFlag-human UBE2B-transfected 293T.Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (98%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%).Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. 靶标信息The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
| 数据： |
| UBE2B Antibody (PA5-21316) in IF Immunofluorescent analysis of UBE2B in paraformaldehyde-fixed A431 cells using a UBE2B polyclonal antibody (Product # PA5-21316) at a 1:500 dilution.UBE2B Antibody (PA5-21316) in WBWestern blot analysis of UBE2B was performed by separating 50 µg of mouse tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a UBE2B Polyclonal Antibody (Product # PA5-21316) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. |
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