LSD1 Polyclonal Antibody
货号：PA5-90711
规格：100 µL
价格：4887
产品类型：标签抗体
品牌：Thermo Fisher
抗原：Recombinant fusion protein containing a sequence c
物种：人/小鼠/大鼠
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 一抗 | 同型对照： | |
| 浓度： | 2.05 mg/mL | 用法： | 1:50-1:100(ICC);1:50-1:100(IF);1:50-1:100(IHC(P));1:500-1:2000(WB) |
产品详细信息Immunogen sequence: LSEDEYYSEE ERNAKAEKEK KLPPPPPQAP PEEENESEPE EPSGVEGAAF QSRLPHDRMT SQEAACFPDI ISGPQQTQKV FLFIRNRTLQ LWLDNPKIQL TFEATLQQLE APYNSDTVLV HRVHSYLERH GLINFGIYKR IKPLPTKKTG KVIIIGSGVS GLAAARQLQS FGMDVTLLEA RDRVGGRVAT FRKGNYVADL GAMVVTGLGG NPMAVVSKQV NMELAKIKQK CPLYEANGQA VPKEKDEMVE Q; Cellular Location: Nucleus靶标信息LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.
| 数据： |
LSD1 Antibody (PA5-90711) in ICCImmunocytochemistry-Immunofluorescence analysis of LSD1 was performed in HeLa cells using LSD1 Polyclonal Antibody (Product # PA5-90711).LSD1 Antibody (PA5-90711) in IHCImmunohistochemistry analysis of LSD1 in paraffin-embedded human esophageal using LSD1 Polyclonal Antibody (Product # PA5-90711) at a dilution of 1:200.LSD1 Antibody (PA5-90711) in WBWestern Blot analysis of LSD1 in extracts of various cell lines using LSD1 Polyclonal Antibody (Product # PA5-90711) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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QQ:1749072012