| The 2',3'-Cyclic GAMP Competitive ELISA Kit quantitates 2',3'-cyclic GAMP in lysed cells and tissue, tissue culture media, and EDTA plasma.|Principle of the method: The 2',3'-Cyclic GAMP Competitive ELISA research-use-only kit is designed to quantitatively measure 2',3'-cyclic GAMP independent of species. A 2',3'-cyclic GAMP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit 2',3'-cyclic GAMP antibodies and 2',3'-cyclic GAMP-peroxidase conjugate is added to the wells. The 2',3'-cyclic GAMP-peroxidase conjugate and any 2',3'-cyclic GAMP in the sample will compete to bind to the anti-rabbit 2',3'-cyclic GAMP antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 2',3'-cyclic GAMP-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 2',3'-cyclic GAMP present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.2'，3'-循环GAMP竞争性ELISA试剂盒对裂解的细胞和组织，组织培养基和EDTA血浆中的2'，3'-循环GAMP进行定量。|方法原理：2'，3'-循环GAMP竞争性ELISA研究专用试剂盒旨在定量测量2'，3'-环状GAMP，而与物种无关。提供2'，3'-环GAMP标准品以生成测定的标准曲线，并从用户生成的标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗兔IgG抗体的透明微量滴定板中。将抗兔2'，3'-环GAMP抗体和2'，3'-环GAMP-过氧化物酶偶联物添加到孔中。样品中的2'，3'-环GAMP-过氧化物酶偶联物和任何2'，3'-环GAMP将竞争结合抗兔2'，3'-环GAMP抗体。孵育后，洗涤板并加入底物。底物与结合的2'，3'-环GAMP-过氧化物酶缀合物反应。孵育30分钟后，停止反应，并在微量滴定板读数器中于450 nm处检测所产生颜色的强度。产生的颜色的强度与样品中存在的2'，3'-环GAMP的数量成反比。|严格的验证：每种ELISA试剂盒的制造批次均经过质量测试，包括敏感性，特异性，精密度和批次间的一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Cyclic GMP-AMP (cGAMP) synthase (cGAS) is a nucleotidyltransferase located in the cytosol that acts as a cytosolic DNA sensor to detect foreign DNA from microbial pathogens as part of the innate immune response. Upon binding to cytosolic DNA, cGAS produces the cyclic dinucleotide second messenger cGAMP, which activates stimulator of interferon genes (STING), leading to activation of the type I interferon (IFN) pathway. In vitro, fibroblasts, macrophages, and dendritic cells isolated from cGAS knockout (cGAS-/-) mice do not produce type I IFNs following DNA transfection or DNA virus infection. Similarly, cells containing a frame-shift mutation in the cGAS locus fail to mount an immune response to HIV and other retroviruses. In vivo, cGAS-/- mice infected with herpes simplex virus 1 (HSV-1) have lower levels of IFN-α and IFN-β, shorter survival times, and higher post-mortem levels of HSV-1 in the brain.环状GMP-AMP（cGAMP）合酶（cGAS）是位于胞质溶胶中的核苷酸转移酶，可作为胞质DNA传感器来检测微生物病原体的外源DNA，作为先天免疫应答的一部分。与细胞质DNA结合后，cGAS产生了环状二核苷酸第二信使cGAMP，它激活了干扰素基因的刺激物（STING），从而激活了I型干扰素（IFN）途径。在体外，从cGAS基因敲除（cGAS-/-）小鼠分离的成纤维细胞，巨噬细胞和树突状细胞在DNA转染或DNA病毒感染后不会产生I型干扰素。同样，在cGAS基因座中包含移码突变的细胞也无法对HIV和其他逆转录病毒产生免疫应答。在体内，感染了单纯疱疹病毒1（HSV-1）的cGAS-/-小鼠大脑中的IFN-α和IFN-β含量较低，存活时间较短，而死后的HSV-1水平较高。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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