GeneJET PCR Purification Kit/GeneJET PCR纯化试剂盒
货号：K0701,K0702
规格：50 preps,250 preps
价格：374,1499
产品类型：PCR 及DNA聚合酶
品牌：Thermo Fisher
| Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 µg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning,in vitrotranscription, blotting orin situhybridization. |
优点： |
| -Highly efficient:90 to 100% recoveries in the range of 100 bp to 10 kb-Fast:procedure takes only 5 minutes-Convenient:spin columns are capped and assembled with collection tubes |
数据： |
| 图1.Effective removal of unincorporated label图2.Purified DNA can be used directly in sequencing reactionsA PCR fragment purified using the GeneJET PCR Purification Kit was sequenced using ABI PRISM® 377 DNA Analyzer. More than 650 nucleotides can be read.图3.Superior purification of DNA fragments from PCR reaction mixture |
组成成分： |
| ▪Binding Buffer▪Wash Buffer (concentrated)▪Elution Buffer (10 mM Tris-HCl, pH 8.5)▪GeneJET Purification Columns (preassembled with
collection tubes) |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:
• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
• In vitro transcription
• In situ hybridization
• Labeling
• Cloning
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