resDNASEQ Human Residual DNA Quantitation Kit/resDNASEQ人残留DNA定量试剂盒

货号：A26366

规格：100 reactions

价格：30597

产品类型：核酸标记

品牌：Thermo Fisher

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of human cell DNA*. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.This kit does not include sample preparation reagents—for this, please seeresDNASEQ Human Residual DNA Quantitation System with PrepSEQ Residual DNA Sample Preparation Kit.• Highly sensitive quantitation using proven TaqMan real-time qPCR technology• Consistent performance across the expected range of DNA fragment sizes• Includes master mix, TaqMan primer/probe mix, and human genomic DNA standardThe resDNASEQ Human Residual DNA Quantitation Kit provides highly sensitive detection of human DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of human DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.Easy to useWith the resDNASEQ Human Residual DNA Quantitation Kit you perform qPCR analysis to compare the amount of human DNA in your test samples to a standard curve generated with known amounts of purified human genomic DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to human genomic DNA standard, as well as step-by-step instructions.Consistent performanceFor accurate quantitation of residual human DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.* Due to the high degree of similarity between genomes, DNA from closely-related primate species such as monkey (Vero), chimpanzee, and orangutan are expected be detected by the resDNASEQ Human Quantitation Kit.

技术参数

组成成分 resDNASEQ™ Human DNA Real-Time PCR Reagents:• 2X Environmental Master Mix (2 tubes, 0.75 mL per tube). Store at -5 to -30°C before first use, protect from light.• 10X Human DNA Assay Mix (1 tube, 300 µL). Store at -5 to -30°C, protect from light.• Negative control water (1 tube, 1.0 mL). Store at -5 to -30°C before first use and 2–8°C after first use.resDNASEQ™ Human DNA Control:• Human DNA Control (1 tube, 40 µL, 30 ng/µL). Store at -5 to -30°C.• DNA Dilution Buffer (1 bottle, 7 mL). Store at -5 to -30°C before first use and 2–8°C after first use.

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