IgD Monoclonal Antibody (10.4.22), Functional Grade, eBioscience™/IgD 单抗 (10.4.22), 功能抗体, eBioscience™
货号:16-5924-82,16-5924-85,16-5924-38,16-5924-025,16-5924-050
规格:100 µg,500 µg,5 mg,25 mg,50 mg
价格:934,1752,24089,72266,120443
产品类型:流式抗体
品牌:eBioscience
抗原:IgD
物种:小鼠
宿主:小鼠
抗体亚型:IgG2a, kappa
克隆号:10.4.22
荧光染料:Alexa Fluor 488
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类型: | 一抗(功能抗体) | 同型对照: | Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™ |
浓度: | 1 mg/mL | 用法: | Assay-Dependent(FN) |
产品详细信息Description: This 10.4.22 monoclonal antibody reacts with the a allotype of mouse IgD, which is expressed by mature peripheral B cells. This antibody has been reported to activate B cells via engagement of the IgD receptor. The use of anti-IgM as a polyclonal B cell activator is problematic due to interference from high levels of circulating serum IgM.Crossblocking studies suggest that the 10.4.22 antibody recognizes a different epitope from 11-26c.Applications Reported: This 10.4.22 antibody has been reported for use in functional assays.Applications Tested: This 10.4.22 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。未经明确授权不得转售。
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参考文献: |
功能检测1.Journal of immunology (Baltimore, Md. : 1950)IL-4 regulates Bim expression and promotes B cell maturation in synergy with BAFF conferring resistance to cell death at negative selection checkpoints."16-5924 was used in Functional assays to analyze the effect of IL-4 on B cell maturation, and its influence on BCR-mediated negative selection."AuthorsGranato A,Hayashi EA,Baptista BJ,Bellio M,Nobrega A |
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