CD4 Monoclonal Antibody (GK1.5), Functional Grade, eBioscience™/CD4 单抗 (GK1.5), 功能抗体, eBioscience™

货号：16-0041-82,16-0041-85,16-0041-86,16-0041-38,16-0041-95,16-0041-025,16-0041-050

规格：100 µg,500 µg,1mg,5mg,10 mg,25 mg,50 mg

价格：1369,2636,4818,24089,29225,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：GK1.5

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activateT cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. The eBioG4.18 antibody has also been used in functional assays.Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD4 Antibody (16-0041-82) in FlowStaining of mouse splenocytes with Anti-Mouse CD4 PE.Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

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参考文献：

流式1Evidence-based complementary and alternative medicine : eCAMBaicalin Downregulates RLRs Signaling Pathway to Control Influenza A Virus Infection and Improve the Prognosis."Published figure using CD4 monoclonal antibody (Product # 16-0041-82) in Flow Cytometry"2.Frontiers in pharmacologyTraditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells."Published figure using CD4 monoclonal antibody (Product # 16-0041-82) in Flow Cytometry"功能检测3.OncoimmunologyTbet and IL-36γ cooperate in therapeutic DC-mediated promotion of ectopic lymphoid organogenesis in the tumor microenvironment."16-0041 was used in in vivo experiments to examine the factors that mediate the formation of ectopic lymphoid organogenesis in the tumour microenvironment."AuthorsWeinstein AM,Chen L,Brzana EA,Patil PR,Taylor JL,Fabian KL,Wallace CT,Jones SD,Watkins SC,Lu B,Stroncek DF,Denning TL,Fu YX,Cohen PA,Storkus WJ4.The Journal of clinical investigationAdipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling."16-0041 was used in Functional assays to demonstrate the importance of ATB2 cells in obesity-induced insulin resistance and suggest that inhibition of the LTB4/LTB4R1 axis might be a useful approach for developing insulin-sensitising therapeutics."AuthorsYing W,Wollam J,Ofrecio JM,Bandyopadhyay G,El Ouarrat D,Lee YS,Oh DY,Li P,Osborn O,Olefsky JMIHC5.Scientific reportsHeavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease."Published figure using CD4 monoclonal antibody (Product # 16-0041-82) in Immunofluorescence"

技术参数

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