RediPlate 96 RiboGreen RNA Quantitation Kit
货号：R32700
规格：1 kit
价格：3040
产品类型：核酸标记
品牌：Thermo Fisher
| The RediPlate 96 RiboGreen RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. |
| 特点 |
| • Automatable–a 10-minute incubation time with no separation steps makes the assay ideal for automated, high-throughput measurements• Precise–the average coefficient of variation for replicate assays is <= 5% using a robotic liquid handling system• Convenient–each plate includes pre-measured RNA standards and is ready for immediate use• Easy to read–the assay can be performed using standard fluorescence-based microplate readers |
| 应用 |
| The RediPlate 96 RiboGreen RNA Quantitation Kit is ideal for measuring yields of RNA preparations for microarray experi-ments, reverse transcription PCR (RT-PCR), differential display PCR, Northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations. |
| 数据 |
Dynamic range and sensitivity of the RediPlate™ 96 RiboGreen® RNA quantitation assay.Dynamic range and sensitivity of the RediPlate™ 96 RiboGreen® RNA quantitation assay. The RNA standards provided in the RediPlate™ 96 RiboGreen® RNA Quantitation Kit (R32700) were added in quadruplicate to assay wells as described in the accompanying protocol, and fluorescence was measured in a fluorescence microplate reader using excitation at 485 ± 12.5 nm and fluorescence detection at 530 ± 15 nm. Fluorescence was plotted against the RNA concentration with no background subtraction. The inset shows the sensitivity of the assay at very low levels of RNA. |
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