SuperScript One-Cycle cDNA Kit for use with Affymetrix One-Cycle Assays
货号:A10752030
规格:30 reactions
价格:40594
产品类型:核酸标记
品牌:Thermo Fisher
The SuperScript One-Cycle cDNA Kit for use with Affymetrix One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperScript II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix’ protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
技术参数 组成成分 50 µM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free WaterAll components are guaranteed stable for 6 months when properly stored.