CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Functional Grade, eBioscience™/CD274 (PD-L1, B7-H1) 单克隆 (MIH5), 功能抗体, eBioscience™

货号：16-5982-81,16-5982-82,16-5982-85,16-5982-38,16-5982-050

规格：50 µg,100 µg,500 µg,5 mg,50 mg

价格：1328,2654,3982,24836,124177

产品类型：流式抗体

品牌：eBioscience

抗原：CD274

物种：人/小鼠

宿主：大鼠

抗体亚型：其它

克隆号：MIH5

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in in vitro and in vivo functional assays.Applications Tested: The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.仅用于科研。不用于诊断过程。

数据

CD274 (PD-L1, B7-H1) Antibody (16-5982-82) in FlowStaining of mouse splenocytes with Anti-Mouse CD274 (B7-H1) PE Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

流式1.Nature communicationsOncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion."Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 16-5982-82) in Flow Cytometry"2.ImmunityOncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA."Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 16-5982-82) in Flow Cytometry"3.ImmunityMaternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission."Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 16-5982-82) in Flow Cytometry"功能检测4.Journal of immunotherapy (Hagerstown, Md. : 1997)Control of established melanoma by CD27 stimulation is associated with enhanced effector function and persistence, and reduced PD-1 expression of tumor infiltrating CD8(+) T cells."16-5982 was used in in vitro experiments to examine the efficacy of agonistic anti-CD27 antibodies as monotherapies for established melanoma in a murine model."AuthorsRoberts DJ,Franklin NA,Kingeter LM,Yagita H,Tutt AL,Glennie MJ,Bullock TN中和5.Nature communicationsNeuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1+Tregcells."16-5982 was used in Neutralization experiments to investigate the role of IFN in generation of FoxA1(+)Treg cells."6.The Journal of experimental medicinePD-L1 is a novel direct target of HIF-1α, and its blockade under hypoxia enhanced MDSC-mediated T cell activation."16-5982 was used in Neutralization experiments to determine that blockage of the HRE within the proximal promoter of PD-1 ligand causes preferential anticancer MDSC determination."AuthorsNoman MZ,Desantis G,Janji B,Hasmim M,Karray S,Dessen P,Bronte V,Chouaib S

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