IL-1 beta Monoclonal Antibody (CRM56), Functional Grade, eBioscience™/IL-1 beta 单克隆 (CRM56),功能抗体, eBioscience™

货号：16-7018-81,16-7018-85

规格：50 µg,500 µg

价格：1196,3389

产品类型：流式抗体

品牌：eBioscience

抗原： IL-1 beta

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：16-7018-025

荧光染料：其它

_ueditor_page_break_tag_

类型:	一抗(功能抗体）	同型对照：	IgG1
浓度：	1 mg/mL	用法：	1-4 µg/mL（ELISA）；Assay-Dependent（中和）；Assay-Dependent（功能检测）

产品详细信息Description: The CRM56 antibody reacts with human and baboon interleukin-1 beta; (IL-1 beta). IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.Applications Reported: The CRM56 antibody has been reported for use in cytokine neutralization, and ELISA. Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry. Functional Grade purified CRM56 antibody, cat. 16-7018, is recommended for use in functional assays.Applications Tested: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1β (IL-1β) in combination with the biotin CRM57 (13-7016) antibody for detection and recombinant human IL-1β (14-8018) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.The Functional Grade Purified CRM56 antibody has been tested by LAL assay to verify low endotoxin and by bioassay to verify neutralization of IL-1β bioactivity. For in vitro neutralization, the CRM56 antibody at 2.5 µg/mL has been found to neutralize by 50% the effect of 1 ng/mL human IL-1β bioactivity in a D10.G4.1 cell proliferation bioassay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.仅用于科研。不用于诊断过程。

数据

IL-1 beta Antibody (16-7018-81)Oncotarget 2015 -Figure 6 Induction of a pro-inflammatory secretory phenotype after CPT treatment of SKOV3 cells A-E. RT-qPCR expression analysis of the indicated pro-inflammatory marker genes at the indicated times of CPT treatment (sample size: n >= 3). FC = 1 (day 0) corresponds to the following Ct values: IL1B: 31.5; IL8: 32; CCL20: 32; CXCL1 : 22; SERPINE1: 26. F. Flow cytometry analysis of intracellular IL-1beta expression after 0, 2 or 21 weeks of CPT treatment (sample size: n = 3). Values represent the fold change (FC) in the number of positive cells relative to small cells on day 0 (normalized to 1). Cells gated for small cells (forward and sideward scatter as untreated SKOV3 cells) and larger cells (forward and sideward scatter larger than for SKOV3 cells). G. Immunoblot analysis of phosphorylated STAT3 in untreated human macrophage cells (negative control), after LPS treatment (positive control), or after an exposure to supernatant from SKOV3 cells treated with CPT for 0, 5 days, 14 days or 21 weeks.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

流式1.Journal of extracellular vesiclesCirculating LL37 targets plasma extracellular vesicles to immune cells and intensifies Behçet's disease severity."Published figure using IL-1 beta monoclonal antibody (Product # 16-7018-81) in Flow Cytometry"2.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsDifferential requirement for P2X7R function in IL-17 dependent vs. IL-17 independent cellular immune responses."16-7018 was used in in vivo experiments to identify agents targeting the P2X7R that might treat Th17-related transplant pathologies, while maintaining Th1-immunity to infection."功能检测3.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsTh17 Responses to Collagen Type V, kα1-Tubulin, and Vimentin Are Present Early in Human Development and Persist Throughout Life."16-7018 was used in Functional assays to investigate the basis of the T helper 17-dependent autoimmune responses associated with fibro-obliterative forms of chronic rejection following heart or lung transplantation."AuthorsSullivan JA,Jankowska-Gan E,Hegde S,Pestrak MA,Agashe VV,Park AC,Brown ME,Kernien JF,Wilkes DS,Kaufman DB,Greenspan DS,Burlingham WJ4.Nature communicationsAdipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate."16-7018 was used in Functional assays to suggest that adipocytes promote malignancy of monocarboxylate transporter 2-expressing breast cancer via Β-hydroxybutyrate potentially by inducing the epigenetic upregulation of tumour-promoting genes."AuthorsHuang CK,Chang PH,Kuo WH,Chen CL,Jeng YM,Chang KJ,Shew JY,Hu CM,Lee WH中和5.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsDifferential requirement for P2X7R function in IL-17 dependent vs. IL-17 independent cellular immune responses."16-7018 was used in in vivo experiments to identify agents targeting the P2X7R that might treat Th17-related transplant pathologies, while maintaining Th1-immunity to infection."

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://www.biorod.cn/thread-26415.htm