IL-33R (ST2) Monoclonal Antibody (RMST2-33), Functional Grade, eBioscience™/IL-33R (ST2) 单克隆 (RMST2-33), 功能抗体, eBioscience™

货号：16-9333-82,16-9333-85,16-9333-38,16-9333-025,16-9333-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：2923,5255,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-33R

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：RMST2-33

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The RMST2-33 monoclonal antibody reacts with mouse ST2, also known as IL-33R or interleukin 1 receptor-like 1. ST2 is a member of the IL-1 receptor family. In the membrane-bound form, it consists of three extracellular immunoglobulin domains and an intracellular toll-interleukin-1 receptor domain. ST2 forms a dimer with IL-1R accessory protein in a ligand-dependent manner. IL-33/ST2 interaction induces the production of TH2 cytokines. ST2 also has a soluble isoform lacking transmembrane and intracellular toll-interleukin-1 receptor domains. It is believed that the soluble form functions as a decoy receptor that can block membrane bound IL-33/ST2 interaction. ST2 is expressed by TH2 lymphocytes, mast cells, eosinophils, basophils, innate lymphocytes, smooth muscle cells, and endothelial cells and is involved in host defense, allergy, and inflammation. IL-33/ST2 interaction has also been shown to be atheroprotective.RMST2-33 has been reported to bind and activate ST2 in in vitro assays.Applications Reported: This RMST2-33 antibody has been reported for use in functional assays and flow cytometric analysis. Functional activity has been determined by the ability to induce IL-6 production in mouse bone marrow-derived mast cells when used between 1-10 µg/mL.Applications Tested: This RMST2-33 antibody has been tested by flow cytometric analysis of bone marrow-derived mast cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5,IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L – the membrane bound form, and ST2S – the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.仅用于科研。不用于诊断过程。

数据

IL-33R (ST2) Antibody (16-9333-82)Scientific reports 2017 -Figure 3 Der f 31 up-regulates the expression levels of TSLP and IL-33 in epithelial cells via TLR2. A549 cells were seeded into 6-well plates overnight and treated with different concentrations of Der f 31. At 4 hours, total RNA was extracted to analyze the expression levels of TSLP and IL-33 by RT-PCR. ( A ) The results of RT-PCR. The lung tissues from normal BALB/c mice were digested, and the cells were incubated with anti-TLR2 antibody, IgG1, kappa isotype control (IC) or TLR4 inhibitor for 2 hours or 6 hours and then treated with Der f 31. At 96 hours, the supernatants were collected to perform ELISA. ( B ) The expression levels of TSLP. ( C ) The expression levels of IL-33. These data (means +- SEMs) were generated from three independent experiments and processed with GraphPad software. The significant differences between two groups were tested by two-tailed T-test. **p < 0.01. ***p < 0.001. ns, no significant difference.

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参考文献：

ELISA1.Scientific reportsDer f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells."Published figure using IL-33R (ST2) monoclonal antibody (Product # 16-9333-82) in ELISA"功能检测2.Nature communicationsHepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance."16-9333 was used in Functional assays to identify strain-specific persistence of Hepatitis B virus, and study the effects of IL-21 and IL-33 on viral clearance in mice."AuthorsShen Z,Yang H,Yang S,Wang W,Cui X,Zhou X,Liu W,Pan S,Liu Y,Zhang J,Zhang J,Xie Y,Liu J

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