IL-13 Monoclonal Antibody (eBio1316H), Functional Grade, eBioscience™/IL-13 单抗 (eBio1316H), 功能抗体, eBioscience™

货号：16-7135-81,16-7135-85,16-7135-38,16-7135-025,16-7135-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1044,3386,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-13

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：eBio1316H

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（ELISA）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The eBio1316H antibody reacts with mouse IL-13. The eBio1316H antibody is a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Ralpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.Applications Reported: The eBio1316H antibody has been reported useful for neutralization, ELISA detection, and ELISPOT detection.Applications Tested: The Functional Grade purified format of the eBio1316H antibody has been tested by LAL assay to verify low endotoxin level and by bioassay to quantitate neutralization of IL-13 biological activity.For in vitro neutralization, the Functional Grade purified eBio1316H antibody at 0.04 µg/mL has been found to neutralize by 50% the biological effect of 7.5 ng/mL mouse IL-13 in an assay of TF-1 cell proliferation. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.仅用于科研。不用于诊断过程。

数据

FIGURE 1/3 IL-13 Antibody (16-7135-81) in ELISAMouse IL-13 ELISPOT: Splenocytes were activated with immobilized Anti-Mouse CD3 (Product # 16-0031-82) and Anti-Mouse CD28 (Product # 16-0281-82) for 24 hrs in a Mouse IL-13 ELISPOT assay (right). Left well is medium alone control (no mitogen). IL-13 Antibody (16-7135-81)Molecular immunology 2011 -Published figure using IL-13 monoclonal antibody (Product # 16-7135-81)

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参考文献：

IF1.Molecular immunologyContribution of IL-13 to early exocrinopathy in Id3-/- mice."Published figure using IL-13 monoclonal antibody (Product # 16-7135-81) in Immunofluorescence"ELISA2. ImmunityPerinatal Activation of the Interleukin-33 Pathway Promotes Type 2 Immunity in the Developing Lung."16-7135 was used in an ELISA assay to suggest enhanced neonatal Th2 cell skewing to inhaled allergens results from postnatal hyperactivity of the IL-33 axis during a period of maximal lung remodeling."Authorsde Kleer IM,Kool M,de Bruijn MJ,Willart M,van Moorleghem J,Schuijs MJ,Plantinga M,Beyaert R,Hams E,Fallon PG,Hammad H,Hendriks RW,Lambrecht BN功能检测3.mAbsDiscovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis."16-7135 was used in Functional assays to identify and characterise a humanised antibody against IL-15."AuthorsVicari AP,Schoepfer AM,Meresse B,Goffin L,Léger O,Josserand S,Guégan N,Yousefi S,Straumann A,Cerf-Bensussan N,Simon HU,Chvatchko Y4.Journal of immunology (Baltimore, Md. : 1950)IL-6 Regulates M2 Polarization and Local Proliferation of Adipose Tissue Macrophages in Obesity."16-7135 was used in Functional assays to study how IL-6 acts as a Th2 cytokine in obesity by stimulating M2 polarisation and local adipose tissue macrophages proliferation."AuthorsBraune J,Weyer U,Hobusch C,Mauer J,Brüning JC,Bechmann I,Gericke M中和5.The Journal of clinical investigationMast cells aggravate sepsis by inhibiting peritoneal macrophage phagocytosis."16-7135 was used in Blocking experiments to investigate the role of resident sentinel cells during the early phase of septic peritonitis."AuthorsDahdah A,Gautier G,Attout T,Fiore F,Lebourdais E,Msallam R,Daëron M,Monteiro RC,Benhamou M,Charles N,Davoust J,Blank U,Malissen B,Launay P6.The American journal of pathologyDual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2."16-7135 was used in Blocking experiments to demonstrate that prostaglandin D2 signals through CRTH2 and DP to mediate contact hypersensitivity."AuthorsYamamoto Y,Otani S,Hirai H,Nagata K,Aritake K,Urade Y,Narumiya S,Yokozeki H,Nakamura M,Satoh T阻断7. Journal of immunology (Baltimore, Md. : 1950)Group 2 Innate Lymphoid Cells Promote an Early Antibody Response to a Respiratory Antigen in Mice."16713581 was used in blocking or activating experiment to suggest that lung group 2 innate lymphoid cells promote B cell production of early antibodies to a respiratory antigen even in the absence of T cells"AuthorsDrake LY,Iijima K,Bartemes K,Kita H

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