Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™/Mouse IgG2a kappa 同型对照 (eBM2a), 功能抗体, eBioscience™

货号：16-4724-82,16-4724-85,16-4724-81,16-4724-38,16-4724-025,16-4724-050

规格：100 µg,500 µg,50 µg,5 mg,25 mg,50 mg

价格：1156,2103,693,24089,72266,120443

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：其它

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类型:	同型对照(功能抗体）	同型对照：
浓度：	1 mg/mL	用法：	Assay-Dependent（Flow）;Assay-Dependent（对照）;Assay-Dependent（功能检测）

产品详细信息Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in bioassays and cell surface and intracellular flow cytometric analysis.Applications Tested: This mouse IgG2a isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. It should be used at the same concentration as the experimental antibody. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

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参考文献：

流式1.Nature methodsChemically defined generation of human cardiomyocytes."16-4724 was used in Flow cytometry/Cell sorting to develop an optimized cardiac differentiation strategy using a chemically defined medium."AuthorsBurridge PW,Matsa E,Shukla P,Lin ZC,Churko JM,Ebert AD,Lan F,Diecke S,Huber B,Mordwinkin NM,Plews JR,Abilez OJ,Cui B,Gold JD,Wu JC2.PloS oneCell surface expression level variation between two common Human Leukocyte Antigen alleles, HLA-A2 and HLA-B8, is dependent on the structure of the C terminal part of the alpha 2 and the alpha 3 domains."16-4724 was used in Flow cytometry/Cell sorting to suggest that the differential cell surface expression of two common HLA-A and-B alleles is regulated by a post-translational mechanism."AuthorsDellgren C,Nehlin JO,Barington T中和3.Thrombosis and haemostasisHigh-mobility group box 1 protein induces tissue factor expression in vascular endothelial cells via activation of NF-kappaB and Egr-1."16-4724 was used in Blocking experiments to delineate the potential role of high-mobility group box 1 protein in the pathogenesis of atherothrombosis."AuthorsLv B,Wang H,Tang Y,Fan Z,Xiao X,Chen F对照4.OncoimmunologyTargeting B-cell malignancies through human B-cell receptor specific CD4+T cells."16-4724 was used as a control in experiments to identify shared BCR-derived CD4+T-cell epitopes with promiscuous HLA DRB1 binding affinity, suggesting a broad strategy to target clonal B cell tumours."AuthorsWeng J,Baio FE,Moriarty KE,Torikai H,Wang H,Liu Z,Maiti SN,Gwak D,Popescu MS,Cha SC,Cooper LJ,Neelapu SS,Kwak LW功能检测5.Clinical cancer research : an official journal of the American Association for Cancer ResearchTargeting human B-cell malignancies through Ig light chain-specific cytotoxic T lymphocytes."16-4724 was used in Functional assays to identify novel immunogenic CTLs epitopes from Id V(L), suggesting that they are naturally presented on the surface of B-cell malignancies, supporting their inclusion in next-generation Id vaccines."AuthorsWeng J,Cha SC,Matsueda S,Alatrash G,Popescu MS,Yi Q,Molldrem JJ,Wang M,Neelapu SS,Kwak LW

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