CD11b Monoclonal Antibody (M1/70), Functional Grade, eBioscience™/CD11b单克隆 (M1/70), 功能抗体, eBioscience™

货号： 16-0112-82,16-0112-85,16-0112-86,16-0112-38,16-0112-025,16-0112-050

规格：100 µg,500 µg,1 mg,5 mg,25 mg,50 mg

价格：1721,3098,5162,24089,72266,113625

产品类型：流式抗体

品牌：eBioscience

抗原：CD11b

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M1/70

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: The M1/70 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections. The M1/70 antibody is not recommended for immunohistochemistry of formalin-fixed paraffin embedded tissue. M1/70 has also been reported in in vitro blocking of CD11b function.Applications Tested: The M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes or bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.仅用于科研。不用于诊断过程。

数据

CD11b Antibody (16-0112-82) in FlowStaining of mouse bone marrow with Anti-Mouse CD11b FITC (left), or PE (right).Appropriate isotype controls were used (open histogram).Cells in the myeloid population were used for anaysis.

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参考文献：

流式1.Frontiers in immunologyTpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells."Published figure using CD11b monoclonal antibody (Product # 16-0112-82) in Flow Cytometry"2.NPJ vaccinesNeutrophil swarming and extracellular trap formation play a significant role in Alum adjuvant activity."Published figure using CD11b monoclonal antibody (Product # 16-0112-82) in Flow Cytometry"IF3.eNeuroContribution of CD137L to Sensory Hypersensitivity in a Murine Model of Neuropathic Pain."Published figure using CD11b monoclonal antibody (Product # 16-0112-82) in Immunofluorescence"4.JCI insightAnalysis of leukocyte transepithelial migration using an in vivo murine colonic loop model."Published figure using CD11b monoclonal antibody (Product # 16-0112-82) in Immunofluorescence"中和5.Nature communicationsMT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis."16-0112 was used in Neutralization experiments to show that MT4-MMP-deficient mice have increased adherence of macrophages to inflamed peritonea, and larger lipid deposits and macrophage burden in atherosclerotic plaques."AuthorsClemente C,Rius C,Alonso-Herranz L,Martín-Alonso M,Pollán Á,Camafeita E,Martínez F,Mota RA,Núñez V,Rodríguez C,Seiki M,Martínez-González J,Andrés V,Ricote M,Arroyo AG

技术参数

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