CD86 (B7-2) Monoclonal Antibody (IT2.2), Functional Grade, eBioscience™/CD86 (B7-2) 单克隆 (IT2.2), 功能抗体, eBioscience™

货号：16-0869-85,16-0869-025,16-0869-050

规格：500 µg,25 mg,50 mg

价格：4085,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD86 (B7-2)

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：IT2.2

荧光染料：其它

_ueditor_page_break_tag_

类型:	一抗(功能抗体）	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）;Assay-Dependent（功能检测）；Assay-Dependent（中和）

产品详细信息Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis. IT2.2 has also been reported in blocking of CD86 in functional studies.Applications Tested: The IT2.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD86 (B7-2) Antibody (16-0869-85) in FlowSurface staining of normal human peripheral blood cells with Anti-Human CD86 (B7-2) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

流式1.Frontiers in pharmacologyGinsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype."Published figure using CD86 (B7-2) monoclonal antibody (Product # 16-0869-81) in Flow Cytometry"2.Oncology lettersPhotodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70."Published figure using CD86 (B7-2) monoclonal antibody (Product # 16-0869-81) in Flow Cytometry"3.Oncology lettersAG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells."Published figure using CD86 (B7-2) monoclonal antibody (Product # 16-0869-81) in Flow Cytometry"ICC4.Biochemistry and biophysics reportsPentraxin-3 regulates the inflammatory activity of macrophages."Published figure using CD86 (B7-2) monoclonal antibody (Product # 16-0869-81) in Immunocytochemistry"中和5.Infection and immunityMycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy."16-0869 was used in Blocking experiments to study how the interplay between M. tuberculosis and NK cells/APC triggering IFN-gamma may play a beneficial role in tuberculous pleurisy by helping to maintain a type 1 profile."AuthorsSchierloh P,Yokobori N,Alemán M,Landoni V,Geffner L,Musella RM,Castagnino J,Baldini M,Abbate E,de la Barrera SS,Sasiain MC

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://www.biorod.cn/thread-26478.htm