CD126 Monoclonal Antibody (D7715A7), Functional Grade, eBioscience™/CD126 单克隆 (D7715A7), 功能抗体, eBioscience™
货号：16-1261-85,16-1261-38,16-1261-025,16-1261-050
规格：500 µg,5 mg,25 mg,50 mg
价格：3624,24089,72266,120443
产品类型：流式抗体
品牌：eBioscience
抗原：CD126
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：D7715A7
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™ |
浓度： | 1 mg/mL | 用法： | 1 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测） |
产品详细信息Description: The monoclonal antibody D7715A7 recognizes mouse CD126, the IL-6 receptor alpha chain, a member of the Ig superfamily. CD126 can heterodimerize with CD130 (gp130) to form the high affinity IL-6 receptor. Once IL-6 is bound, signaling from the CD130 subunit is initiated and the receptor complex is internalized. The IL-6 receptor is also reported to exist as a soluble version, which bound to IL-6 acts in trans to signal. Expression is found on T lymphocytes in the periphery and duringdevelopment in the thymus is restricted to SP populations, unlike gp130 which is expressed in all thymocytes. Additional expression has been shown on activated B lymphocytes, macrophages, granulocytes and CD34+ hematopoietic progenitor cells. The monoclonal antibody D7715A7 binding can be blocked addition of ligand (IL-6).Applications Reported: This D7715A7 antibody has been reported for use in flow cytometric analysis and has also been reported to block cytokine binding and inhibit in vitro and in vivo growth of cells dependent on IL-6.Applications Tested: This D7715A7 antibody has been tested by blocking of direct conjugate when staining mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin 6 receptor (IL6R), also known as CD126 (Cluster of Differentiation 126), is a type I cytokine receptor. This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.仅用于科研。不用于诊断过程。
CD126 Antibody (16-1261-85) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.5 µg of Rat IgG2b kappa Isotype Control Purifiedl (Product # 14-4031-82) (left) or 0.5 µg of Anti-Mouse CD126 Functional Grade Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.
二抗
荧光二抗
免疫组化一抗
标签抗体
流式
1.Nature immunologyLEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6."16-1261 was used in Flow cytometry/Cell sorting to report that selective loss of Lef1 or Tcf7 results in T(FH) cell defects."AuthorsChoi YS,Gullicksrud JA,Xing S,Zeng Z,Shan Q,Li F,Love PE,Peng W,Xue HH,Crotty S中和
2.PLoS pathogensTLR4 and NKT cell synergy in immunotherapy against visceral leishmaniasis."16-1261 was used in Neutralization experiments to develop a defined, immunotherapeutic strategy for the treatment of immune diseases using a mouse model of visceral leishmaniasis."AuthorsKarmakar S,Bhaumik SK,Paul J,De T
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