CD39 Monoclonal Antibody (24DMS1), Functional Grade, eBioscience™/CD39单克隆 (24DMS1), 功能抗体, eBioscience™

货号：16-0391-83,16-0391-025,16-0391-050

规格：200 µg,25 mg,50 mg

价格：4556,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD39

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：24DMS1

荧光染料：其它

类型:	一抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 488, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent(FN);1:250(IF);0.06µg/test（Flow）;0.06µg/test（WB）;Assay-Dependent(对照)

产品详细信息Description: The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, another E-NTPase. Together, these molecules influence inflammation responses. CD39 is expressed on B cells, Langerhans cells and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.Applications Reported: This 24DMS1 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).Applications Tested: This 24DMS1 antibody has been tested by flow cytometric analysis of mouse splenocytes and by WB. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.This antibody is provided as a functional grade but has not been tested in functional assay yet.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.仅用于科研。不用于诊断过程。

数据

CD39 Antibody (16-0391-38) in WBCell lysates prepared from mouse spleen (lane 1), thymocytes (lane 2), or bone marrow (lane 3) were resolved by SDS-PAGE prior to transfer onto a PVDF membrane.The membrane was probed with 2 µg/mL Anti-Mouse CD39 Purified and bands were detected using Anti-Rat IgG HRP.Note: The Anti-Mouse CD39 Purified works only with non-reduced protein without boiling prior to gel loading.

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参考文献：

WB1.Frontiers in immunologyToll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation."Published figure using CD39 monoclonal antibody (Product # 16-0391-83) in Flow Cytometry"Flow2.Frontiers in immunologyToll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation."Published figure using CD39 monoclonal antibody (Product # 16-0391-83) in Flow Cytometry"3.Purinergic signallingSelective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis."Published figure using CD39 monoclonal antibody (Product # 16-0391-83) in Flow Cytometry"4.Cell stem cellCD150highBone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine."Published figure using CD39 monoclonal antibody (Product # 16-0391-83) in Flow Cytometry"5.PloS oneSequential induction of effector function, tissue migration and cell death during polyclonal activation of mouse regulatory T-cells."16-0391 was used in Flow cytometry/Cell sorting to investigate the origin and fate of IL-10 producing Tregs."AuthorsLangenhorst D,Gogishvili T,Ribechini E,Kneitz S,McPherson K,Lutz MB,Hünig T

技术参数

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