Hydrazide-AMCA (hydrazide-activated aminomethylcoumarin acetate)/酰肼-AMCA(酰肼活化的氨基甲基香豆素乙酸酯)
货号:33015
规格:5 mg
价格:0
产品类型:蛋白标记
品牌:Thermo Fisher
AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye with reactive derivatives that are used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke's shift and resistance to photobleaching. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC. AMCA has an excitation maximum at 345nm and an emission maximum at 450nm. |
特点 |
•AMCA—activated derivatives of blue-fluorescent aminomethylcoumarin acetate dye•Amine-specific labeling—NHS-ester and Sulfo-NHS-ester varieties of AMCA efficiently label antibodies and other purified proteins at primary amines (lysine side chains)•Glycoprotein labeling—hydrazide variety of AMCA labels oxidized sugar groups in glycoproteins or other carbohydrates |