Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7
货号：25-4301-81,25-4301-82
规格：50μg,100μg
价格：1244,1577
产品类型：大鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：大鼠
抗体亚型：IgG1, kappa
荧光染料：PE-Cy7
| 抗体类型 | 大鼠同型对照 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | 1 µg/test (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG1 K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
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| 参考文献： |
| 1.PloS oneHeterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines."25-4301 was used in Flow cytometry to investigate the potential of a previously uncharacterized property of natural human adenovirus infection to dictate, modulate and/or alter the course of HCV infection upon exposure."AuthorsSingh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B |
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