IgM Monoclonal Antibody (II/41), APC-eFluor 780
货号：47-5790-82
规格：100μg
价格：2630
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：II/41
荧光染料：APC-eFluor 780
| 抗体类型 | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780 |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. |
| 数据 |
| IgM Antibody (47-5790-82) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.25 µg of Anti-Mouse IgM APC-eFluor® 780 (right). Cells in the small scatter population were used for analysis. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.Proceedings of the National Academy of Sciences of the United States of AmericaLigase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining."Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry"2.ImmunityNuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation."Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry"3.BloodA critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation."Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry"4.Cell regeneration (London, England)A protocol for generating induced T cells by reprogramming B cellsinvivo."Published figure using IgM monoclonal antibody (Product # 47-5790-82) in Flow Cytometry"5.Cell reportsLineage-Specific Genes Are Prominent DNA Damage Hotspots during Leukemic Transformation of B Cell Precursors."47-5790 was used in Flow cytometry/Cell sorting to demonstrate lineage-specific transcription predisposes genes in B cells to DNA damage."AuthorsBoulianne B,Robinson ME,May PC,Castellano L,Blighe K,Thomas J,Reid A,Müschen M,Apperley JF,Stebbing J,Feldhahn N |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://www.biorod.cn/thread-26767.htm
QQ:1749072012