CD2 Monoclonal Antibody (RPA-2.10), PE, eBioscience™

货号：12-0029-42

规格：100tests

价格：2271

产品类型：流式抗体

品牌：Thermo Fisher

抗原： CD2

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：RPA-2.10

荧光染料：PE

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.RPA-2.10 crossreacts to non-human primates and pigs.Applications Reported: The RPA-2.10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD2 Antibody (12-0029-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human/Non-Human Primate CD2 PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyA monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.2.Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay."12-0029 was used in Flow cytometry/Cell sorting to describe a targeted bioluminescence resonance energy transfer (BRET) assay, called type-4 BRET, which detects both homo- and heteromeric interactions using induced multimerization of protomers."3.Proceedings of the National Academy of Sciences of the United States of AmericaSignaling pathways activated by a protease allergen in basophils."12-0029 was used in Flow cytometry/Cell sorting to study signaling pathways activated by a protease allergen in basophils."4.Molecular and cellular biologyForced expression of cyclin-dependent kinase 6 confers resistance of pro-B acute lymphocytic leukemia to Gleevec treatment."12-0029 was used in Flow cytometry/Cell sorting to find that forced expression of Cdk6 promotes continued cell division and decreased Gleevec-induced apoptosis of leukaemic cells."

技术参数

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