DyLight 650-4xPEG NHS 酯-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

DyLight 650-4xPEG NHS 酯

2024-10-18

DyLight 650-4xPEG NHS 酯

货号：62274

规格：1 mg

价格：4783

产品类型：蛋白标记

品牌：Thermo Fisher

Thermo Scientific DyLight 650-4xPEG 胺反应性染料是我们的高性能DyLight 650 染料的衍生物，可用于对抗体和其他蛋白进行荧光标记，进而用于远红外检测。DyLight 650-4xPEG 染料包含无细胞毒性的 4 聚乙二醇 (PEG) 链，可增强荧光并减少由其制成的偶联物的非特异性结合。由 DyLight 650-4xPEG 染料制成的偶联物可以用作细胞成像和其他荧光检测方法的 Molecular Probes。DyLight 650-4xPEG 染料的远红外到 NIR 荧光特性使其特别适用于多种生物、化学和制药应用，包括体内成像。PEG 链还可以提高染料和标记分子在水溶液中的溶解度，有助于对细胞的通透性并改善组织保留。

特点

•高荧光强度—相对于 Alexa Fluor，荧光显著更明亮647•聚乙二醇化—提高在水溶液中的溶解度，有助于对细胞的通透性

应用

•荧光显微镜检查•体内或离体成像•基于细胞的检测•流式细胞术/荧光活化细胞分选 (FACS)

数据

DyLight 650-4xPEG Dye is compatible with flow cytometersThe LFA1 cell surface adhesion glycoprotein in Jurkat cells was fluorescently labeled with mouse anti-LFA1 primary antibody and DyLight 650-4xPEG Dye-conjugated goat anti-mouse secondary antibody. The stained cells were then counted using the BD™ Accuri™ C6 Flow Cytometer.

DyLight 650-4xPEG Dye exhibits rapid biodistribution and tissue clearanceMice were injected with hydrolyzed DyLight 650-4xPEG or DyLight 650 (non-PEGylated) Dye via the retro-orbital plexus, and NIR in vivo images were captured pre (Pre)- and post-injection at the indicated time points (top images). Data indicate that both dyes are rapidly distributed throughout the mouse body, but the PEGylated dye is more rapidly cleared than the non-PEGylated dye. After the final time point, animals were sacrificed and tissues collected for ex vivo imaging of the kidneys and GI tract to indicate clearance of the dyes via the excretory system. Heat map legends indicate signal intensity (bottom panel).

技术参数

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