FOXP3 Monoclonal Antibody (236A/E7), PE, eBioscience™

货号：12-4777-42,12-4777-41

规格：100 Tests,25 Tests

价格：3118,1474

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：236A/E7

荧光染料：PE

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test

产品详细信息Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and IHC.Applications Tested: This 236A/E7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (12-4777-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) (left) and Anti-Human CD25 APC (Product # 17-0259-42) (right) followed by fixation and permeabilization using the Foxp3 Staining/Transcription Factor Buffer Set (Product # 00-5523-00) and intracellular staining with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human Foxp3 PE. Cells in the lymphocyte gate were used for analysis.FOXP3 Antibody (12-4777-42)Intracellular staining of human peripheral blood cells. As expected based on known expression patterns, Foxp3 clone 236A/E7 stains CD4+CD25+ T cells and does not stain CD4- cells or CD4+CD25- T cells. Details: Normal human peripheral blood cells were surface stained with CD4 (clone OKT4) and CD25 (clone BC96) followed by intracellular staining with Foxp3 (clone 236A/E7) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD4- (left) and CD4+ (right) gates were used for analysis.

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-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献

1.OncoimmunologyTadalafil has biologic activity in human melanoma. Results of a pilot trial with Tadalafil in patients with metastatic Melanoma (TaMe)."12-4777 was used in Flow cytometry/Cell sorting to evaluate biologic effects, safety and efficacy of palliative treatment with the PDE-5 inhibitor tadalafil in metastatic melanoma patients."AuthorsHassel JC,Jiang H,Bender C,Winkler J,Sevko A,Shevchenko I,Halama N,Dimitrakopoulou-Strauss A,Haefeli WE,Jäger D,Enk A,Utikal J,Umansky V2.Experimental hematology and oncologyChanges in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation withJAK2V617Fallelic burden.AuthorsKovacsovics-Bankowski M,Kelley TW,Efimova O,Kim SJ,Wilson A,Swierczek S,Prchal J3.Frontiers in immunologyATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells."12-4777 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment."AuthorsLecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A4.GutDifferential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo."12-4777 was used in Flow cytometry/Cell sorting to analyse the homing of effector and regulatory T-cells to the inflamed gut via α4β7 and GPR15."AuthorsFischer A,Zundler S,Atreya R,Rath T,Voskens C,Hirschmann S,López-Posadas R,Watson A,Becker C,Schuler G,Neufert C,Atreya I,Neurath MF5.Cancer researchThe CD4(+) T-cell response of melanoma patients to a MAGE-A3 peptide vaccine involves potential regulatory T cells."12-4777 was used in Immunocytochemistry to investigate the CD4+ T-cell response of melanoma patients to a MAGE-A3 peptide vaccine."AuthorsFrançois V,Ottaviani S,Renkvist N,Stockis J,Schuler G,Thielemans K,Colau D,Marchand M,Boon T,Lucas S,van der Bruggen P

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