| 产品详细信息Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and, in low levels, monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 1.The Journal of allergy and clinical immunologyRegulatory T cells produce profibrotic cytokines in the skin of patients with systemic sclerosis."46-0047 was used in Flow cytometry/Cell sorting to determine whether Treg cells in the blood and skin of patients with SSc acquired abnormal production of effector cytokines."AuthorsMacDonald KG,Dawson NAJ,Huang Q,Dunne JV,Levings MK,Broady R2.The Journal of clinical investigationAlloantigen-specific regulatory T cells generated with a chimeric antigen receptor."46-0047 was used in Flow cytometry/Cell sorting to investigate alloantigen-specific regulatory T cells generated with a chimeric antigen receptor."AuthorsMacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK3.Cancer cellEnhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting."46-0047 was used in Flow cytometry/Cell sorting to investigate whether chimeric antigen receptor anti-CD19-engineering of the CD1d-restricted invariant natural killer T cells would result in enhanced anti-lymphoma activity."AuthorsRotolo A,Caputo VS,Holubova M,Baxan N,Dubois O,Chaudhry MS,Xiao X,Goudevenou K,Pitcher DS,Petevi K,Kachramanoglou C,Iles S,Naresh K,Maher J,Karadimitris A4.Nature communicationsT cell receptor β-chains display abnormal shortening and repertoire sharing in type 1 diabetes."46-0047 was used in Flow cytometry/Cell sorting to suggest that short CDR3s increase the potential for self-recognition, conferring heightened risk of autoimmune disease."AuthorsGomez-Tourino I,Kamra Y,Baptista R,Lorenc A,Peakman M5.The Journal of clinical investigationAlloantigen-specific regulatory T cells generated with a chimeric antigen receptor."46-0047 was used in Flow cytometry/Cell sorting to investigate alloantigen-specific regulatory T cells generated with a chimeric antigen receptor."AuthorsMacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK |
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