| 产品详细信息Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969).Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometic analysis of PHA stimulated human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 1.Journal of immunology researchLipopolysaccharide Increases Immune Activation and Alters T Cell Homeostasis in SHIVB'WHU Chronically Infected Chinese Rhesus Macaque."12-2799 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind lipopolysaccharide disruption of immune responses in Chinese rhesus macaques."2.Journal of virologyIncreased frequency of regulatory T cells accompanies increased immune activation in rectal mucosae of HIV-positive noncontrollers."12-2799 was used in Flow cytometry/Cell sorting to investigate the role of Tregs in persistent HIV infection, showing that an increased frequency of Tregs accompanies increased immune activation in HIV-positive noncontrollers."3.Scientific reportsTumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets."12-2799 was used in Flow cytometry/Cell sorting to study the regulation of immune function-related genes in human T cell subsets through tumor-derived exosomes."4.iScienceYY1Upregulates Checkpoint Receptors and Downregulates Type I Cytokines in Exhausted, Chronically Stimulated Human T Cells."12-2799 was used in Flow cytometry/Cell sorting to elucidate a Yin Yang-1-centred mechanism for diverse cellular components correlated with exhaustion."5.Science advancesImmune evasion mediated by PD-L1 on glioblastoma-derived extracellular vesicles."12-2799 was used in Flow cytometry/Cell sorting to investigate whether glioblastoma extracellular vesicles are important mediators of immunosuppression and whether programmed death ligand-1 could play a role."AuthorsRicklefs FL,Alayo Q,Krenzlin H,Mahmoud AB,Speranza MC,Nakashima H,Hayes JL,Lee K,Balaj L,Passaro C,Rooj AK,Krasemann S,Carter BS,Chen CC,Steed T,Treiber J,Rodig S,Yang K,Nakano I,Lee H,Weissleder R,Breakefield XO,Godlewski J,Westphal M,Lamszus K,Freeman GJ,Bronisz A,Lawler SE,Chiocca EA |
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