Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor 532
货号：58-4732-81
规格：50 µg
价格：1595
产品类型：流式抗体
品牌：Thermo Fisher
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
荧光染料：Alexa Fluor 532
| 抗体类型 | 单抗 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.Applications Reported: This eBMG2b antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBMG2b antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. Use at the same concentration as the experimental antibody.Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. Thiscannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 数据 |
| Mouse IgG2b kapa Isotype Control (58-4732-81) in FlowNormal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and staining buffer (autofluorescence) (left) or 0.125 µg of Mouse IgG2b kappa Isotype Control, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis. |
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