Rat IgG1 kappa Isotype Control (eBRG1)
货号：14-4301-82,14-4301-85
规格：100 μg,500 μg
价格：934,1394
产品类型：大鼠同型对照
品牌：eBioscience
物种：其它
宿主：大鼠
抗体亚型：IgG1, kappa
荧光染料：其它
| 抗体类型 | 同型对照 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | Assay-Dependent (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and immunoglobulin ELISA.Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 数据 |
| Rat IgG1 kappa Isotype Control (14-4301-82) in IHC (F)Immunohistochemistry of frozen mouse intestine stained with 2.5 µg/mL Rat IgG1 K Isotype Control Purified (left) or 2.5 µg/mL Anti-Mouse Lyve-1 Purified (right) followed by 5 µg/mL Anti-Rat IgG1 eFluor® 615. Nuclei are stained with DAPI. |
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| 参考文献： |
| 1.Journal of immunology (Baltimore, Md. : 1950)Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+Dendritic Cells at the Site of Infection."14-4301 was used in Immunocytochemistry-immunoflourescence to present Toxoplasma infection as a novel in vivo murine model to study the immunomodulatory properties of free ISG15 and uniquely link it to IL-1Β production by CD8α+ dendritic cells driven by two cysteines in the hinge region of the protein."2.Journal of immunology (Baltimore, Md. : 1950)Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+Dendritic Cells at the Site of Infection."14-4301 was used in Immunocytochemistry-immunoflourescence to present Toxoplasma infection as a novel in vivo murine model to study the immunomodulatory properties of free ISG15 and uniquely link it to IL-1Β production by CD8α+ dendritic cells driven by two cysteines in the hinge region of the protein."3.Frontiers in immunologyTrichinella spiralisInfection Mitigates Collagen-Induced ArthritisviaProgrammed Death 1-Mediated Immunomodulation."14-4301 was used in Flow cytometry/Cell sorting to introduce the first evidence that PD-1 plays a critical role in Trichinella spiralis infection-attenuated collagen-induced arthritis in a mouse model by regulating the CD4+ T cell function, which may provide the new insights into the mechanisms of helminth-induced immunomodulation of host autoimmunity."4.Epigenetics & chromatinEstablishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points."14-4301 was used in Flow cytometry/Cell sorting to demonstrate that megakaryopoietic profiles are established early in haematopoiesis and are present in the majority of the haematopoietic progenitor population."5.The Journal of clinical investigationA TLR9-dependent checkpoint governs B cell responses to DNA-containing antigens."14-4301 was used in Flow cytometry/Cell sorting to describe a relationship among the B cell receptor, TLR9, and cytokine signals that regulate B cell responses to DNA-containing antigens." |
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