CD4 Monoclonal Antibody (GK1.5), PerCP-eFluor 710

货号：46-0041-80,46-0041-82

规格：25 ug,100 µg

价格：1164,2794

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：GK1.5

荧光染料：PerCP-eFluor™ 710

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor 710
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (46-0041-80) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD3 eFluor® 450 (Product # 48-0032-82) and 0.06 µg of Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.06 µg of Anti-Mouse CD4 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis. CD4 Antibody (46-0041-80)Staining of mouse splenocytes. As expected based on known relative expression patterns, CD4 clone GK1.5 stains about 25% of total lymphocytes and does not stain CD45R (B220)+ B cells. Details: Balb/c splenocytes were surface stained with CD45R (B220, clone RA3-6B2) and Rat IgG2b Isotype Control or CD4 (clone GK1.5). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine ResearchHigh-dose interferon-γ promotes abortion in mice by suppressing Treg and Th17 polarization.2. Nature communicationsTreg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.3.Investigative ophthalmology & visual scienceSuppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2.4. Nature communicationsA cancer vaccine-mediated postoperative immunotherapy for recurrent and metastatic tumors.5. CirculationMyocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes.

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