CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine7, eBioscience™

货号：25-0691-82,25-0691-81

规格：100 µg,50 µg

价格：1880,1311

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：H1.2F3

荧光染料：PE-Cy7

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™
浓度：	0.5mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: The H1.2F3antibody has been reported for use in flow cytometric analysis.Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。

数据

CD69 Antibody (25-0691-82) in FlowStaining of ConA-stimulated splenocytes with Anti-Mouse CD69 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)mTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function."25-0691 was used in Flow cytometry/Cell sorting to investigate whether mTORC1-controlled metabolism underpins normal natural killer cell proinflammatory functions."AuthorsDonnelly RP,Loftus RM,Keating SE,Liou KT,Biron CA,Gardiner CM,Finlay DK2.European journal of immunologyNitric oxide inhibits the accumulation of CD4+CD44hiTbet+CD69lo T cells in mycobacterial infection."25-0691 was used in Flow cytometry/Cell sorting to support the hypothesis that effector T cells in mycobacterial granulomata are not a uniform effector population but exist in distinct subsets with differential susceptibility to the regulatory effects of nitric oxide."AuthorsPearl JE,Torrado E,Tighe M,Fountain JJ,Solache A,Strutt T,Swain S,Appelberg R,Cooper AM3.The Journal of experimental medicineDendritic cell sphingosine-1-phosphate lyase regulates thymic egress."25-0691 was used in Flow cytometry/Cell sorting to investigate the role of dendritic cell sphingosine-1-phosphate lyase in thymic egress."AuthorsZamora-Pineda J,Kumar A,Suh JH,Zhang M,Saba JD4.The Journal of experimental medicineNKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20."25-0691 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells."AuthorsDrennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D5.Stem cell reportsMFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation."25-0691 was used in Flow cytometry/Cell sorting to elucidate the molecules and mechanisms pertinent to the low immunogenicity of undifferentiated embryonic stem cells."

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