CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), APC, eBioscience™

货号：17-0626-82,17-0626-80

规格：100 µg,25 µg

价格：3157,1526

产品类型：流式抗体

品牌：eBioscience

抗原：CD62p

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：Psel.KO2.3

荧光染料：APC

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25µg/test（Flow）

产品详细信息Description: The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (also known as P-selectin and GMP-140), a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that isconserved across several species (e.g. mouse, human, and rat) and has not been reported to block cell adhesion.Applications Reported: This Psel.KO2.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This Psel.KO2.3 antibody has been tested by flow cytometric analysis on thrombin-activated human platelets. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes tothe plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.仅用于科研。不用于诊断过程。

数据

CD62P (P-Selectin) Antibody (17-0626-82) in FlowStaining of thrombin-activated human platelets with Anti-Human CD41a FITC (Product # 11-0419-42) and 0.125 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or 0.125 µg of Anti-Human/Mouse CD62P (P-selectin) APC (right). Total viable cells were used for analysis.

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参考文献：

1.Journal of hematology & oncologyNovel antibodies against GPIbα inhibit pulmonary metastasis by affecting vWF-GPIbα interaction."Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 17-0626-82) in Flow Cytometry"2.Cell researchCirculating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors."Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 17-0626-82) in Flow Cytometry"3.PloS oneCathepsin G-dependent modulation of platelet thrombus formation in vivo by blood neutrophils."Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 17-0626-82) in Flow Cytometry"4.American journal of physiology. Renal physiologyPlatelets are relevant mediators of renal injury induced by primary endothelial lesions."17-0626 was used in Flow cytometry/Cell sorting to assess the abilities of various modified platelets to undergo relevant activation after the induction of selective endothelial injury."AuthorsSchwarzenberger C,Sradnick J,Lerea KM,Goligorsky MS,Nieswandt B,Hugo CP,Hohenstein B5.The Journal of clinical investigationPlatelet-derived HMGB1 is a critical mediator of thrombosis."17-0626 was used in Flow cytometry/Cell sorting to investigate the contribution of platelet-derived HMGB1 in thrombosis, showing that HMGB1 is a critical mediator of thrombosis, suggesting that it could be a target for treatment for abnormal coagulation disorders."

技术参数

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