CD27 Monoclonal Antibody (LG.7F9), APC, eBioscience™

货号：17-0271-82,17-0271-81

规格：100 µg,50 µg

价格：2895,1416

产品类型：流式抗体

品牌：eBioscience

抗原：CD27

物种：人/小鼠/大鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：LG.7F9

荧光染料：APC

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™
浓度：	0.2 mg/mL	用法：	0.06 µg/test（Flow）

产品详细信息Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis.Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.仅用于科研。不用于诊断过程。

数据

CD27 Antibody (17-0271-82) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.03 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (left) or 0.03 µg of Anti-CD27 APC (right). Total viable cells were used for analysis.

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参考文献：

1. Cell reportsIterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells.AuthorsVink CS,Calero-Nieto FJ,Wang X,Maglitto A,Mariani SA,Jawaid W,Göttgens B,Dzierzak EYear2020SpeciesMouse2. Journal of virologyBroadening CD4+and CD8+T Cell Responses against Hepatitis C Virus by Vaccination with NS3 Overlapping Peptide Panels in Cross-Priming Liposomes."17-0271 was used in Flow cytometry/Cell sorting to develop a novel peptide-based nanoparticle vaccine strategy aimed at generating T cell immunity against potential subdominant T cell epitopes that are not efficiently targeted by vaccination with full-length recombinant protein or infection with Hepatitis C virus."AuthorsFilskov J,Mikkelsen M,Hansen PR,Christensen JP,Thomsen AR,Andersen P,Bukh J,Agger EM3.The Journal of experimental medicineNKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20."17-0271 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells."AuthorsDrennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D4. Journal of virologyPlasmid vector-linked maturation of natural killer (NK) cells is coupled to antigen-dependent NK cell activation during DNA-based immunization in mice."17-0271 was used in Flow cytometry/Cell sorting to analyze the mechanisms underlying NK cell activation, and their potential role in adaptive immunity during DNA-based immunization in mice."AuthorsZhu R,Mancini-Bourgine M,Zhang XM,Bayard F,Deng Q,Michel MLYear2011SpeciesMouse5. Nature immunologyIL-1β-driven neutrophilia preserves antibacterial defense in the absence of the kinase IKKβ."17-0271 was used in Flow cytometry/Cell sorting to observe neutrophilia in mice with an inducible deletion of IKKβ (Ikkβ(δ) mice) which is believed to be critical for innate immunity."AuthorsHsu LC,Enzler T,Seita J,Timmer AM,Lee CY,Lai TY,Yu GY,Lai LC,Temkin V,Sinzig U,Aung T,Nizet V,Weissman IL,Karin M

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