CD28 Monoclonal Antibody (37.51), PE,

货号：12-0281-81,12-0281-82

规格：50μg ,100μg

价格：1029,2019

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：37.51

荧光染料：PE

类型:	单抗	同型对照：	Syrian Hamster IgG Isotype Control, PE
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (12-0281-81) in FlowSwiss Webster mouse splenocytes were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0031-82) and 0.25 µg of Armenian Hamster IgG Isotype Control, PE (Product # 12-4888-83) (left) or 0.25 µg of CD28 Monoclonal Antibody, PE (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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参考文献

1. Shock (Augusta, Ga.)Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.2. Journal of immunology (Baltimore, Md. : 1950)Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.3. Nature communicationsThe ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.4. Journal of immunology (Baltimore, Md. : 1950)IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.5. Journal of immunology (Baltimore, Md. : 1950)Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.

技术参数

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