CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), Alexa Fluor 488
货号：53-0083-81
规格：100 µg
价格：1195
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：Alexa Fluor 488
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 488 |
| 浓度： | 0.5 mg/mL | 用法： | 0.25 μg/test (Flow)； |
| 产品详细信息Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. |
| 数据 |
| CD8b Antibody (53-0083-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.125 µg of Rat IgG2b K Isotype Control Alexa Fluor® 488 (Product # 53-4031-80) (left) or 0.125 µg of Anti-Mouse CD8b Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Microbial pathogenesisCD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice.2.Infection and immunityDepletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease.AuthorsTarleton RL,Sun J,Zhang L,Postan M3.Neurobiology of diseasePeripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice."Published figure using CD8b monoclonal antibody (Product # 53-0083-82) in Flow Cytometry"4.Nature communicationsIntratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity."Published figure using CD8b monoclonal antibody (Product # 53-0083-82) in Flow Cytometry"5.Nature communicationsMetastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression."Published figure using CD8b monoclonal antibody (Product # 53-0083-82) in Flow Cytometry" |
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