CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), APC
货号：17-0083-81
规格：50 µg
价格：1577
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：APC
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), APC |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: APC Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 数据 |
| CD8b Antibody (17-0083-81) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.25 µg of Anti-Mouse CD8b APC. Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Molecular therapy oncolyticsIntratumoral INF-γ triggers an antiviral state in GL261 tumor cells: a major hurdle to overcome for oncolytic vaccinia virus therapy of cancer."17-4031 was used in Flow cytometry/Cell sorting to identify microenvironmental factors, which may be decisive for failure or success of oncolytic VACV therapy."2.Clinical and vaccine immunology : CVIInteractive changes between macrophages and adipocytes."17-4031 was used as a Control in experiments to determine whether communication between macrophages and adipocytes disrupts their function and affects insulin resistance."AuthorsXie L,Ortega MT,Mora S,Chapes SK3.Thrombosis and haemostasisFactor VIII delivered by haematopoietic stem cell-derived B cells corrects the phenotype of haemophilia A mice."17-4031 was used in Flow cytometry/Cell sorting to provide evidence for B cell-directed protein delivery as a potential clinical approach to treat haemophilia A and other similar disorders."AuthorsRamezani A,Zweier-Renn LA,Hawley RG4.BMC immunologyCd1d regulates B cell development but not B cell accumulation and IL10 production in mice with pathologic CD5(+) B cell expansion."17-4031 was used in Flow cytometry/Cell sorting to investigate the effect of CD1d expression on CD5+ B cell accumulation, IL10 competence and antibody production in mice with pathologic CD5+ B cell expansion."AuthorsPalmer VL,Nganga VK,Rothermund ME,Perry GA,Swanson PC5.Stem cells and developmentEnrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19."17-4031 was used in Flow cytometry/Cell sorting to provide the first evidence that s-SHIP-GFP promoter reporter offers a unique marker for the enrichment of human stem-like cell populations."AuthorsBauderlique-Le Roy H,Vennin C,Brocqueville G,Spruyt N,Adriaenssens E,Bourette RP |
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