| 产品详细信息Description: The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in the thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA has been implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4^+ and CD8^+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes. It is not understood why there is a discrepancy in thymocyte staining however clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7 in splenocytes and bone marrow cells.Applications Reported: This 6F7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 6F7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily. BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells, but not Th2 cells. BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM). This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response. |
| 1.European journal of microbiology & immunologyImpact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function."12-5950 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules."AuthorsAutengruber A,Gereke M,Hansen G,Hennig C,Bruder D2.Proceedings of the National Academy of Sciences of the United States of AmericaB and T lymphocyte attenuator inhibits LPS-induced endotoxic shock by suppressing Toll-like receptor 4 signaling in innate immune cells."12-5950 was used in Flow cytometry/Cell sorting to elucidate the role of BTLA expressed on innate cells for controlling overwhelming immune responses."AuthorsKobayashi Y,Iwata A,Suzuki K,Suto A,Kawashima S,Saito Y,Owada T,Kobayashi M,Watanabe N,Nakajima H3.PLoS pathogensHTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors."12595082 was used in flow cytometry to assess whether human T-cell leukemia virus type 1 bZIP factor enhances the proliferation of expressing T cells after stimulation via the T-cell receptor"AuthorsKinosada H,Yasunaga JI,Shimura K,Miyazato P,Onishi C,Iyoda T,Inaba K,Matsuoka M4.European journal of microbiology & immunologyImpact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function."12-5950 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules."AuthorsAutengruber A,Gereke M,Hansen G,Hennig C,Bruder D5.Journal of immunology (Baltimore, Md. : 1950)IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency."12-5950 was used in Flow cytometry/Cell sorting to investigate modulation of hepatitis-inducing T-cell responses by the coinhibitor herpes virus entry mediator."AuthorsWahl C,Wegenka UM,Leithäuser F,Schirmbeck R,Reimann J |
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