CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE, eBioscience™

货号：12-1541-83,12-1541-82,12-1541-81

规格：200 µg,100 µg,50 µg

价格：2892,2055,1029

产品类型：流式抗体

品牌：eBioscience

抗原：CD154

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：MR1

荧光染料：PE

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.Applications Reported: The MR1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.仅用于科研。不用于诊断过程。

数据

CD154 (CD40 Ligand) Antibody (12-1541-83) in FlowT cells sorted from BALB/c splenocytes using the MagniSort™ Mouse T cell Enrichment Kit (Product # 8804-6820-74) were unstimulated (orange histogram) or stimulated with the Cell Stimulation Cocktail (Product # 00-4970-03) (purple histogram) and stained with 0.125 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left) or 0.125 µg of Anti-Mouse CD154 (CD40 Ligand) PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Arthritis research & therapyInterleukin-21 signaling in B cells, but not in T cells, is indispensable for the development of collagen-induced arthritis in mice."12-1541 was used in Flow cytometry/Cell sorting to study the role of IL-21 signalling in B and T cells in collagen-induced arthritis."AuthorsSakuraba K,Oyamada A,Fujimura K,Spolski R,Iwamoto Y,Leonard WJ,Yoshikai Y,Yamada HYear2016SpeciesMouse 2. BMC biologyElongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile."12-1541 was used in Flow cytometry/Cell sorting to study how the conformation of TCR alpha chain CDR3 favours different CD4 cytokine profiles."AuthorsReynolds C,Chong D,Raynsford E,Quigley K,Kelly D,Llewellyn-Hughes J,Altmann D,Boyton RYear2014SpeciesMouse 3. Immune networkDetection of Foreign Antigen-specific CD4(+)Foxp3(+) Regulatory T Cells by MHC Class II Tetramer and Intracellular CD154 Staining."12-1541 was used in Flow cytometry/Cell sorting to evaluate the use of intracellular CD154 staining to detect and characterize foreign Ag-specific CD4+Foxp3+ regulatory T-cells in various models."AuthorsChoi JY,Eo SKYear2013SpeciesMouse 4. Journal of immunology (Baltimore, Md. : 1950)IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells."12-1541 was used in Flow cytometry/Cell sorting to investigate the role of germinal centres in autoimmunity, showing that IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells."AuthorsDing Y,Li J,Wu Q,Yang P,Luo B,Xie S,Druey KM,Zajac AJ,Hsu HC,Mountz JDYear2013SpeciesMouse 5. Journal of thrombosis and haemostasis : JTHPlatelet shedding of CD40L is regulated by matrix metalloproteinase-9 in abdominal sepsis."12-1541 was used in Flow cytometry/Cell sorting to investigate whether matrix metalloproteinase-9 cleaves surface-expressed CD40L and regulates pulmonary accumulation of neutrophils in sepsis."AuthorsRahman M,Zhang S,Chew M,Syk I,Jeppsson B,Thorlacius H

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