CD20 Monoclonal Antibody (2H7), APC, eBioscience

货号：17-0209-42

规格：100 Tests

价格：2629

产品类型：流式抗体

品牌：eBioscience

抗原：CD20

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：2H7

荧光染料：APC

抗体类型:	人CD20细胞流式抗体	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), APC, eBioscience
浓度：	5 μL/Test	用法：	5μL（0.06 µg）/test (Flow)

Description:The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD20 Antibody (17-0209-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human CD20 APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.PloS oneAn efficient low cost method for gene transfer to T lymphocytes."17-0209 was used in Flow cytometry/Cell sorting to describe a novel protocol for electroporation based modification of T lymphocytes."2.PLoS pathogensDecreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration."17-0209 was used in Flow cytometry/Cell sorting to test the efficacy of IL-7 adjunctive therapy on T-cell reconstitution in antiretroviral therapy-suppressed HIV patients."3.Histochemistry and cell biologyMultispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs."17-0209 was used in Flow cytometry/Cell sorting to provide a solid basis for better insight into the function of tetraspanins in the human immune response."4.Cell reportsUTX/KDM6A Loss Enhances the Malignant Phenotype of Multiple Myeloma and Sensitizes Cells to EZH2 inhibition."17-0209-41 was used in flow cytometry as a surface marker to determine difference in cells +/- UTX expression."

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