CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE
货号：12-9942-81,12-9942-82
规格：100μg,100μg
价格：1164,2000
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：PE
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), PE |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 μg/test(Flow) |
| 产品详细信息Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.Applications Reported: The 7E.17G9 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. |
| 数据 |
| CD278 (ICOS) Antibody (12-9942-81) in FlowStaining of unstimulated (left) and 3-day Con A-stimulated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) PE (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 660, were used for analysis. |
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| 参考文献： |
| 1.Journal of autoimmunityICOS expression by effector T cells influences the ability of regulatory T cells to inhibit anti-chromatin B cell responses in recipient mice."12-9942 was used in Flow cytometry/Cell sorting to study the effect of elevated effector T-cell ICOS levels on immunity and immune cells."2.The Journal of experimental medicineIFN-γ- and IL-10-expressing virus epitope-specific Foxp3(+) T reg cells in the central nervous system during encephalomyelitis."12-9942 was used in Flow cytometry/Cell sorting to show that pathogen epitope-specific Foxp3(+) T reg cells can be identified on the basis of cytokine production."3.OncotargetAmphiregulin activates regulatory T lymphocytes and suppresses CD8+ T cell-mediated anti-tumor response in hepatocellular carcinoma cells."12-9942 was used in Flow cytometry/Cell sorting to investigate how hepatocellular carcinoma cells alter endogenous anti-tumor immunity and their related signaling pathways."4.European journal of immunologyEfficient help for autoreactive B-cell activation requires CD4+ T-cell recognition of an agonist peptide at the effector stage."12-9942 was used in Flow cytometry/Cell sorting to investigate the effect of stimulation by agonist or partial agonist ligands during initial CD4(+) T-cell priming on antibody production by anti-chromatin B cells."5.Nature immunologyFoxp3 and Toll-like receptor signaling balance Tregcell anabolic metabolism for suppression."12-9942 was used in Flow cytometry/Cell sorting to demonstrate that inflammatory signals and Foxp3 balance mTORC1 signaling and glucose metabolism to control the proliferation and suppressive function of Treg cells." |
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