CD21 Monoclonal Antibody (HB5), APC, eBioscience™

货号：17-0219-42

规格：100 tests

价格：2987

产品类型：流式抗体

品牌：eBioscience

抗原：CD21

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：HB5

荧光染料：APC

抗体类型:	人CD21细胞流式抗体	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™
浓度：	5 μL/Test	用法：	5μL（0.03 µg）/test (Flow)

Description:This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: The APC conjugated mouse IgG2a isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at less than or equal to 1 µg/million cells (100 µL blood). It is recommended thatthe antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD21 Antibody (17-0219-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0038-80) and Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or Anti-Human CD21 APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.European journal of immunologyLong-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers."Published figure using CD21 monoclonal antibody (Product # 17-0219-42) in Flow Cytometry"2.Journal of immunology (Baltimore, Md. : 1950)Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion."Published figure using CD21 monoclonal antibody (Product # 17-0219-42) in Flow Cytometry"3.The Journal of experimental medicineAtypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies."17-0219 was used in Flow cytometry/Cell sorting to show that Plasmodium falciparum (Pf) infection induces the development of memory B cells that produce antibodies against blood stage Pf parasites."4.The Journal of experimental medicineAtypical and classical memory B cells produce Plasmodium falciparum neutralizing antibodies."17-0219 was used in Flow cytometry/Cell sorting to show that Plasmodium falciparum (Pf) infection induces the development of memory B cells that produce antibodies against blood stage Pf parasites."

技术参数

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