CD23 Monoclonal Antibody (EBVCS2), APC-eFluor 780, eBioscience™
货号：47-0238-42
规格：100 Tests
价格：3202
产品类型：流式抗体
品牌：eBioscience
抗原：CD23
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号： EBVCS2
荧光染料：APC-eFluor 780
| 类型: | 人B细胞流抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780, eBioscience™ |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.125 μg)/test(Flow) |
产品详细信息Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23.Applications Reported: This EBVCS2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood andtonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
| CD23 Antibody (47-0238-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD23 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.ImmunologyInterleukin-25 fails to activate STAT6 and induce alternatively activated macrophages."Published figure using CD23 monoclonal antibody (Product # 47-0238-42) in Flow Cytometry" |
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