IgM Monoclonal Antibody (II/41), Biotin,

货号：13-5790-82,13-5790-85

规格：100 ug,500 ug

价格：1237,1649

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：Biotin

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Biotin
浓度：	0.5 mg/mL	用法：	0.25 µg/test(Flow)；Assay-Dependent(IHC (P))；Assay-Dependent(ICC/IF)

产品详细信息Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use inflow cytometric analysis, immunohistochemistry, and immunocytochemistry.Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (13-5790-82) in FlowStaining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a kappa Isotype Control Biotin (Product # 13-4321-82) (left) or 0.06 µg of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)A p53 axis regulates B cell receptor-triggered, innate immune system-driven B cell clonal expansion.2. CirculationFatty Acid Metabolic Defects and Right Ventricular Lipotoxicity in Human Pulmonary Arterial Hypertension.3. The Journal of experimental medicineElucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.4. Journal of immunology (Baltimore, Md. : 1950)A p53 axis regulates B cell receptor-triggered, innate immune system-driven B cell clonal expansion.5. Cell reportsChronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.

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