Human anti Human actin beta antibody
货号：VMA00014
规格：100μl
价格：2890
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：actin beta
物种：人
宿主：其它
抗体亚型：其它
荧光染料：Purified
| 产品介绍 | | |
| Human anti Human actin beta antibodyrecognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modelling.Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant speciesArabidopsis thaliana(mouse eared cress) ACT1 (Arabidopsis) gene products.Actin beta is a commonly used western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms.Human anti Human actin beta antibody recognizes human actin beta as a single band of ~42 kDa in multiple human and rodent cell line lysates by western blotting under reducing conditions. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed inE. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid. |
| Preparation | Recombinant human monoclonal antibody purified by metal chelate affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Purified human beta actin |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories.This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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技术参数 产品应用 wb
产品类型 单克隆抗体
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