| 产品介绍 | | |
| Recombinant human CD120bis an 18.9 kDa peptide comprised of 174 amino acids representing the soluble form of CD120b. Soluble human CD120b, otherwise known as sTNF receptor type II (sTNFRII), is a member of the tumour necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE.CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFRII acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Preparation | Purified recombinant CD120b expressed inE.coli |
| Reconstitution | Reconstitute with 0.2 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Activity | Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.125 µg/ml. |
| Preservative Stabilisers | None present |
| Approx. Protein Concentrations | 1.0 mg/ml after reconstitution |
| Protein Molecular Weight | 18.9 kDa (174 amino acid residues) |
| Purity | >98% by SDS PAGE and HPLC |
| Regulatory | For research purposes only |
| Guarantee | 3 months from date of reconstitution |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
QQ:1749072012