| 产品介绍 | | |
| Recombinant Adiponectinproducted in Hi5 cells derived from the cabbage looper mothTrichoplusia nicorresponds to amino acids 19-244 of the human adiponectin precurson and containing an N-terminal histidine tag. Adiponectin is an adipokine exclusively secreted by adipocytes, which plays a major role in the control of fat metabolism and insulin sensitivity, through the regulation of energy homeostasis and glucose and lipid metabolism. A reduced level of plasma adiponectin is associated with type 2 diabetes and obesity insulin resistance. Adiponectin is an abundant protein in normal serum corresponding to approximately 0.1% of the total protein content. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Preparation | Purified recombinant adiponectin expressed in High-5 insect cells using a baculovirus expression system |
| Reconstitution | Reconstitute with 25 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Buffer Solution | 10mM TRIS, pH 8.575mM L-Arginine |
| Activity | Determined by a cytotoxic assay using M1 cells. The ED50for this effect is 3.0-6.0 μg/ml. |
| Preservative Stabilisers | None present |
| Protein Molecular Weight | 26 kDa (250 amino acid residues) |
| Purity | >97% by SDS PAGE and HPLC |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Prior to reconstitution store at -20℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
QQ:1749072012